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pact2 prey vector  (TaKaRa)


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    Structured Review

    TaKaRa pact2 prey vector
    Pact2 Prey Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prey+vector+pact2/pm38763272-45-12-15
    Average 86 stars, based on 1 article reviews
    pact2 prey vector - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    cDNA Library Assay:

    Article Title: A novel EB-1/AIDA-1 isoform, AIDA-1c, interacts with the Cajal body protein coilin
    Article Snippet: .. A human brain cDNA library cloned into the prey vector pACT2 and pretransformed into the yeast strain Y187 (Clontech) was mated with the strain PJ69-2A harboring the bait vector pAS2-1-coilin(362–576, C214) per the manufacturer's instructions. ..

    Clone Assay:

    Article Title: A novel EB-1/AIDA-1 isoform, AIDA-1c, interacts with the Cajal body protein coilin
    Article Snippet: .. A human brain cDNA library cloned into the prey vector pACT2 and pretransformed into the yeast strain Y187 (Clontech) was mated with the strain PJ69-2A harboring the bait vector pAS2-1-coilin(362–576, C214) per the manufacturer's instructions. ..

    Article Title: Studying Coronavirus–Host Protein Interactions
    Article Snippet: Saccharomyces cerevisiae YRG-2 strain, Mata ura 3-52 his 3-200 ade 2-101 lys 2-801 trp 1-901 leu 2-3 112 gal 4-542 gal 80-538 LYS2::UAS GAL1 -TATA GAL1 -HIS3 URA3::UAS GAL4 17mers (x3)-TATA CYC1 -lacZ. pBD-GAL4 Cam phagemid vector (Agilent technology). pAD-GAL4-2.1 Amp phagemid vector (Agilent technology). pACT2 (Clontech). .. For library-based screening, tissues-specific cDNA libraries cloned into prey vector pACT2 (Clontech). ..

    Plasmid Preparation:

    Article Title: A novel EB-1/AIDA-1 isoform, AIDA-1c, interacts with the Cajal body protein coilin
    Article Snippet: .. A human brain cDNA library cloned into the prey vector pACT2 and pretransformed into the yeast strain Y187 (Clontech) was mated with the strain PJ69-2A harboring the bait vector pAS2-1-coilin(362–576, C214) per the manufacturer's instructions. ..

    Article Title: Characterization of the Src/Abl Hybrid Kinase SmTK6 of Schistosoma mansoni
    Article Snippet: .. As prey vector pACT2 (leucine nutritional marker LEU2, GAL4-AD; Clontech) was used, and pBridge as bait vector was used. ..

    Article Title: TEM8 marks neovasculogenic tumor-initiating cells in triple-negative breast cancer
    Article Snippet: The intracellular domain of human TEM8 ORF was cloned into donor vector pGBKT7 (Clontech Laboratories) to generate the bait plasmid, which expresses TEM8 intracellular domain (icd) protein fused to amino acids 1–147 of the GAL4 DNA binding domain (DNA-BD). .. The prey vector pACT2 (Clontech Laboratories) containing human cDNA collections in-frame fused to the GAL4 activating domain (AD). ..

    Article Title: Studying Coronavirus–Host Protein Interactions
    Article Snippet: Saccharomyces cerevisiae YRG-2 strain, Mata ura 3-52 his 3-200 ade 2-101 lys 2-801 trp 1-901 leu 2-3 112 gal 4-542 gal 80-538 LYS2::UAS GAL1 -TATA GAL1 -HIS3 URA3::UAS GAL4 17mers (x3)-TATA CYC1 -lacZ. pBD-GAL4 Cam phagemid vector (Agilent technology). pAD-GAL4-2.1 Amp phagemid vector (Agilent technology). pACT2 (Clontech). .. For library-based screening, tissues-specific cDNA libraries cloned into prey vector pACT2 (Clontech). ..

    Article Title: veA -dependent RNA-pol II transcription elongation factor like protein, RtfA, is associated with secondary metabolism and morphological development in Aspergillus nidulans
    Article Snippet: Detection was carried out using enhanced chemiluminescence substrate (Amersham, USA). .. Yeast-two hybrid system The plasmid pAMC46, containing veA in the bait vector pGBKT7 (Clonetech), and pEE1 plasmid, containing kapA in the prey vector pACT2 (Clonetech), were used as positive control in this experiment, and empty vectors were used as negative controls as previously described ( Stinnett et al., 2007 ). ..

    Marker:

    Article Title: Characterization of the Src/Abl Hybrid Kinase SmTK6 of Schistosoma mansoni
    Article Snippet: .. As prey vector pACT2 (leucine nutritional marker LEU2, GAL4-AD; Clontech) was used, and pBridge as bait vector was used. ..

    Library Screening:

    Article Title: Studying Coronavirus–Host Protein Interactions
    Article Snippet: Saccharomyces cerevisiae YRG-2 strain, Mata ura 3-52 his 3-200 ade 2-101 lys 2-801 trp 1-901 leu 2-3 112 gal 4-542 gal 80-538 LYS2::UAS GAL1 -TATA GAL1 -HIS3 URA3::UAS GAL4 17mers (x3)-TATA CYC1 -lacZ. pBD-GAL4 Cam phagemid vector (Agilent technology). pAD-GAL4-2.1 Amp phagemid vector (Agilent technology). pACT2 (Clontech). .. For library-based screening, tissues-specific cDNA libraries cloned into prey vector pACT2 (Clontech). ..

    Positive Control:

    Article Title: veA -dependent RNA-pol II transcription elongation factor like protein, RtfA, is associated with secondary metabolism and morphological development in Aspergillus nidulans
    Article Snippet: Detection was carried out using enhanced chemiluminescence substrate (Amersham, USA). .. Yeast-two hybrid system The plasmid pAMC46, containing veA in the bait vector pGBKT7 (Clonetech), and pEE1 plasmid, containing kapA in the prey vector pACT2 (Clonetech), were used as positive control in this experiment, and empty vectors were used as negative controls as previously described ( Stinnett et al., 2007 ). ..



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    Image Search Results


    Plasmids used in this study

    Journal: The Journal of Biological Chemistry

    Article Title: N-terminal methionine excision of proteins creates tertiary destabilizing N-degrons of the Arg/N-end rule pathway

    doi: 10.1074/jbc.RA118.006913

    Figure Lengend Snippet: Plasmids used in this study

    Article Snippet: Y2H screening and binding assays To screen human UBR1-binding proteins, a human pancreas cDNA library cloned into the prey pACT2 vector (638820, Clontech) was transformed into S. cerevisiae strain pJ69 l4A expressing a bait pCH1591 (expressing human UBR1 1–1031 ) using the lithium-acetate protocol ( 61 ).

    Techniques: Plasmid Preparation, Marker